Journal: Materials Today Bio

Article Title: Hepatocyte growth factor in biosheets promotes autonomous regeneration of cutaneous tissue after transplantation onto a full-thickness skin defect

doi: 10.1016/j.mtbio.2026.102969

Figure Lengend Snippet: Analysis of surface markers and multipotency of stromal cells in biosheets (A) Representative flow cytometry profiles of cells isolated from 2w-biosheets and identification of a population of quadruple-positive cells (P7). (B) The ratio of quadruple-positive cells (P7) to stromal cells (P5) in the biosheets and fascia. (C-E) Representative images of alkaline phosphatase (ALP) staining and Alizarin red staining (C), oil red staining (D), Toluidine blue (TB) staining and Safranine O (SO) staining (E) of stromal cells isolated from biosheets and subjected to osteogenic, adipogenic and chondrogenic differentiation. (F-H) Expression levels of osteogenic (F), adipogenic (G), and chondrogenic (H) marker genes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The cells formed pellets after 3 days of culture, and the medium was replaced with MSC Chondrogenic Differentiation Medium (C-28012, PromoCell).

Techniques: Flow Cytometry, Isolation, Staining, Expressing, Marker

Journal: Materials Today Bio

Article Title: Kartogenin-loaded chitosan composite scaffold with cartilage-mimetic microstructure for layered osteochondral repair and cartilage phenotype maintenance

doi: 10.1016/j.mtbio.2025.102727

Figure Lengend Snippet: In vitro chondrogenic activity and cartilage matrix formation in CSK@P100, CSK@P200, CSK@P300, and CSK@P/H100 groups. (A) Safranin O staining after 24 days of culture on CSK@P100, CSK@P200, CSK@P300, and CSK@P/H100 surfaces. (B) Alcian Blue staining at the bottom of culture plates after 24 days of co-culture with CSK@P100, CSK@P200, CSK@P300, and CSK@P/H100. (C–E) mRNA expression levels of Prg4 , Col2a1 , and Col10a1 after 7 and 14 days of chondrogenic induction. (n = 3, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Article Snippet: The cell-scaffold constructs were cultured in rBMSC chondrogenic differentiation medium (Solarbio, Beijing, China) for 24 days.

Techniques: In Vitro, Activity Assay, Staining, Co-Culture Assay, Expressing

Journal: Materials Today Bio

Article Title: Kartogenin-loaded chitosan composite scaffold with cartilage-mimetic microstructure for layered osteochondral repair and cartilage phenotype maintenance

doi: 10.1016/j.mtbio.2025.102727

Figure Lengend Snippet: Evaluation of chondrogenic and ossification of the superficial layer by IF staining and RT-qPCR analysis of different groups after 12 weeks of surgery. (A) IF staining of Collagen II (red). (B) IF staining of Collagen X (red). (C) Semi-quantitative analysis of fluorescence intensity. (D) Quantitative RT-qPCR analysis of Prg4 and Col10a1 gene expression in newly formed cartilage layer. (n = 3, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, scale bars: 50 μm).

Article Snippet: The cell-scaffold constructs were cultured in rBMSC chondrogenic differentiation medium (Solarbio, Beijing, China) for 24 days.

Techniques: Staining, Quantitative RT-PCR, Fluorescence, Gene Expression

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Meniscus progenitor cells combined with joint lavage promote meniscus regeneration and cartilage protection in rat models

doi: 10.3389/fbioe.2025.1724656

Figure Lengend Snippet: IL-1β suppresses the chondrogenic differentiation and migration of MPCs. Untreated and IL-1β-treated MPCs were subjected to (A,D) osteogenic, (B,E) adipogenic, and (C,F) chondrogenic differentiation assays. IL-1β enhanced osteogenic differentiation but markedly inhibited chondrogenic matrix formation. (G) Representative scratch assay images showing reduced cell migration following IL-1β treatment. (H) Quantification of wound healing rates confirms significantly impaired migratory capacity. Scale bar: 200 μm. *P < 0.05, **P < 0.01.

Article Snippet: After 21 days of differentiation, lipid droplets were visualized by staining with Oil Red O solution (Procell) for 30 min. To assess the chondrogenic potential of the cells, untreated and IL-1β-treated MPCs (4 × 10 5 cells) were pelleted by centrifugation (250 × g for 5 min) and cultured in chondrogenic differentiation medium (Procell, PD-015) according to the manufacturer’s instructions.

Techniques: Migration, Wound Healing Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Meniscus progenitor cells combined with joint lavage promote meniscus regeneration and cartilage protection in rat models

doi: 10.3389/fbioe.2025.1724656

Figure Lengend Snippet: IL-1β alters the gene expression profile of MPCs. (A) Flow cytometry analysis shows representative expression patterns of stem/progenitor surface markers in MPCs. Quantitative real-time PCR reveals significant downregulation of chondrogenic genes (B–E) and upregulation of inflammatory genes (F–J) following IL-1β exposure. ns: not significant, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: After 21 days of differentiation, lipid droplets were visualized by staining with Oil Red O solution (Procell) for 30 min. To assess the chondrogenic potential of the cells, untreated and IL-1β-treated MPCs (4 × 10 5 cells) were pelleted by centrifugation (250 × g for 5 min) and cultured in chondrogenic differentiation medium (Procell, PD-015) according to the manufacturer’s instructions.

Techniques: Gene Expression, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

Journal: Journal of Orthopaedic Translation

Article Title: VEGF-neutralized platelet-rich plasma with adipose-derived stromal vascular fraction enhanced osteochondral repair in a point-of-care goat model ☆

doi: 10.1016/j.jot.2025.101034

Figure Lengend Snippet: Live/Dead assay. (A-L) To evaluate viability of ASCs encapsulated in PXL hydrogel, Live/Dead assay was performed on cultured samples in chondrogenic medium at day 3, day 7, and day 21. (M) Live cells and dead cells on day 21 samples were counted using Image J Fiji, and there was no significant difference between groups in calculated cell viability.

Article Snippet: Trilineage differentiation of passage 3 goat ASCs in monolayer was performed in the appropriate differentiation medium: (1) chondrogenic medium (DMEM, 1 % v/v antibiotic-antimycotic, 10 μg/mL insulin-transferrin-selenium (ITS; Invitrogen, Carlsbad, CA), 0.1 μM dexamethasone, 40 μg/mL proline, 50 μg/mL ascorbic acid, and 10 ng/mL recombinant human transforming growth factor-β3 (PeproTech #100-36E, Rocky Hill, NJ, USA); (2) osteogenic medium (DMEM, 10 % v/v FBS, 1 % v/v antibiotic-antimycotic, 0.1 μM dexamethasone, 10 mM β-glycerophosphate, and 50 μg/mL ascorbic acid); and (3) adipogenic medium (DMEM, 10 % v/v FBS, 1 % v/v antibiotic-antimycotic, 1 μg/mL ITS, 1 μM dexamethasone, and 0.5 mM 3-isobutyl-1-methylxanthine (IBMX)).

Techniques: Live Dead Assay, Cell Culture